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KMID : 1007520130220051367
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Food Science and Biotechnology
2013 Volume.22 No. 5 p.1367 ~ p.1373
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Purification and Characterization of A Novel Skatole-degrading Protease from Lactobacillus brevis 1.12
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Meng Xiao
He Zhi-Fei
Li Hong-Jun
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Abstract
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Skatole, a substance with an unpleasant flavor, is released from the cecum and colon of entire male pigs. In this study, a novel skatole-degrading protease from the fermentation broth of Lactobacillus brevis 1.12 was extracted and purified through (NH4)2SO4 precipitation and chromatography with DEAE-Sephadex A-25 and Sephadex G-75. The purified protease was found to be homogeneous in terms of SDS-PAGE and had a molecular mass estimate of 57.5 kDa. This skatole-degrading protease had an optimal pH of 5.0 and optimal temperature of 35oC. The enzyme was only inhibited greatly at a 10 mmol 1/L concentration of K+. The results suggest that the novel skatole-degrading protease was purified. The biochemical characteristics of this protease can provide useful information for its potential use in industrial applications.
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KEYWORD
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skatole-degrading protease, Lactobacillus brevis 1.12, purification, characterizatio
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